Fast Decalcifier
In clinical and research experiments, decalcifying bone samples is the first step in testing bone samples. Effectively removing calcium from the bones while preserving the biological substances within tissue cells, including DNA, RNA, and proteins, is crucial for the success of subsequent molecular extraction and in situ detection of the samples. PhenoVision Biotechnology Co., Ltd. in Beijing has introduced a U.S.-licensed RNA protection formula and developed a rapid decalcifying solution. This solution contains a mixture of acids, stabilizers, and other auxiliary components. It can rapidly decalcify bone samples while preserving the DNA, RNA, and proteins within the cells of the bone samples, ensuring successful follow-up testing.
Classification:
Reagent
Product accessories:
In clinical and research experiments, decalcifying bone samples is the first step in testing bone samples. Effectively removing calcium from the bones while preserving the biological substances within tissue cells, including DNA, RNA, and proteins, is crucial for the success of subsequent molecular extraction and in situ detection of the samples. PhenoVision Biotechnology Co., Ltd. in Beijing has introduced a U.S.-licensed RNA protection formula and developed a rapid decalcifying solution. This solution contains a mixture of acids, stabilizers, and other auxiliary components. It can rapidly decalcify bone samples while preserving the DNA, RNA, and proteins within the cells of the bone samples, ensuring successful follow-up testing.
1. Rapid Decalcification: Most bone samples can be decalcified within 12 to 24 hours.
2. Sample Protection: Preserves DNA, RNA, and protein components within the cells of bone samples.
3. Good Staining Results: Minimizes impact on tissue, ensuring optimal staining outcomes.
|
Product Name |
Item No. |
Specification (ml) |
Save condition |
Validity Period |
|
Fast Decalcifier |
PVB-3001 |
100 |
Preserve at room temperature |
12 months |
|
PVB-3002 |
500 |
Preserve at room temperature |
12 months |
|
|
PVB-3003 |
5000 |
Preserve at room temperature |
12 months |
After 22 hours of treatment with the Fast Decalcifier, mouse teeth were prepared into paraffin sections for RNA ISH detection. The red signal dots in the figure represent the positive RNA signals detected.
Human bone marrow samples were processed with Fast Decalcifier and prepared into paraffin sections for RNA ISH detection. The white, red, and green signal spots in the image represent three different positive RNA signals detected.
Human bone marrow samples were processed with Fast Decalcifier and prepared into paraffin sections for H&E staining.
Human bone marrow samples were processed with Fast Decalcifier and prepared into paraffin sections for mlF detection. The red, green, yellow, and white signals in the image represent positive immunofluorescence staining results for CD68, CD20, CD31, and CD11c, respectively.
In clinical and scientific experiments, decalcification of bone samples is the first step in the detection of bone samples. How to effectively remove the calcium component in the bone and retain the biological substances in the tissue cells, including DNA, RNA and protein, is related to the success or failure of the subsequent molecular extraction and in situ detection of the sample.
Beijing finowaikang technology co., ltd. has introduced a special formula for RNA protection authorized by the United States. the rapid decalcification solution developed includes mixed proportioning acid, stabilizer and other auxiliary components. on the basis of rapid decalcification, DNA,RNA and protein in bone sample cells can be preserved to the greatest extent to ensure subsequent detection.
The product has the following characteristics:
rapid decalcification:3 to 48 hours to complete decalcification of most bone samples
Protection sample:Maximum protection of intracellular DNA, RNA and protein components in bone samples
dyeing effect is good:Small impact on the tissue, to ensure the dyeing effect
Mouse teeth were prepared into paraffin sections after 22 hours of rapid decalcification solution treatment, and RNAISH detection was carried out. The red signal point in the figure is the positive RNA signal detected.
Human bone marrow samples were treated with rapid decalcification solution and prepared into paraffin sections for H & E staining.
Human bone marrow samples were prepared into paraffin sections after rapid decalcification solution treatment, and RNA ISH detection was carried out. White, red and green signal points in the figure are three different positive RNA signals detected.
Human bone marrow samples were prepared into paraffin sections after rapid decalcification solution treatment, and mlF detection was carried out. The red, green, yellow and white signals in the figure were CD68, CD20, CD31 and CD11C immunofluorescence staining positive results respectively.
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