Beijing Fino Weikang Biotechnology Co., Ltd.

PVB Gill1 Hematoxylin

PVB Gill 1 Hematoxylin is a nuclear staining solution prepared using a formula authorized in the United States. The positively charged aluminum-oxidized hematoxylin complex binds with the negatively charged phosphate groups of nuclear DNA to form a blue-violet precipitate.

Classification:

Reagent

Product accessories:

Product Description
Operation steps
Case Presentation

Product Introduction

Product name：PVB Gill1 Hematoxylin
Cat：PVB-3006-001(500mL)
　　 PVB-3006-002(1000mL)
preservation condition：Store at room temperature away from light for 12 months

Product Description

PVB Gill 1 Hematoxylin is a nuclear staining solution prepared using a formula authorized in the United States. The positively charged aluminum-oxidized hematoxylin complex binds with the negatively charged phosphate groups of nuclear DNA to form a blue-violet precipitate.

This product is suitable for immunohistochemical nuclear staining (blue) in formalin-fixed, paraffin-embedded tissue sections, frozen sections, and cytological preparations. It can be used for manual operations as well as for nuclear staining in immunohistochemistry on various fully automated staining machines. Additionally, it is applicable for nuclear staining using RNAscope chromogenic assay kits (brown and red).

Precautions

1.Please pay attention to personal protection during the use of this reagent product, including wearing lab coats, gloves, masks, and other protective equipment. For more details, refer to the SDS (Safety Data Sheet).
2.Use the diluted product as soon as possible to avoid affecting the staining effect.
3.Dispose of both used and unused solutions in accordance with the relevant regulations of your locality, state, and government.
4.Contains sodium iodate, which may cause allergic reactions.

Method of application

Paraffin, Frozen, and Cytology Samples
1.Immunohistochemical (IHC) Procedure and DAB Staining;
2.Rinse 5 times with distilled water or deionized water;
3.Stain with PVB Gill 1 Hematoxylin Solution for 0.5–2 minutes (staining time needs to be optimized according to the type of automated machine); rinse 5 times with distilled water or deionized water;
4.Blueing for 15–60 seconds; rinse 5 times with distilled water or deionized water;
5.Dehydrate and mount the slides.

Color rendering results

The PVB Gill1 hematoxylin stains the nucleus into blue.